CM 8th Semester CM 803 Bio-Process Technology Syllabus RGPV / RGTU B.E. Chemical Engineering(CM) VIII-8th Semester Syllabus

RGTU/RGPV Bio-Process Technology Syllabus
CM 803 Bio-Process Technology Syllabus
Chemical Engineering(CM) VIII-8th Semester Syllabus

CM 803 Bio-Process Technology Course Contents:

Unit I Introduction to Bio-Chemical Engineering: Aspects of microbiology, cell theory structureof microbial cells, classification of microorganism, Essential chemicals of life lipids, Sugars and Polysaccharides, RNA and DNA, Amino acids and proteins.

Unit II Metabolism and Energetic: Assimilatory and dissimilatory process, metabolic mechanism of the cells; Biochemical Kinetics: Simple enzyme kinetics with one or two substrates, modulation and regulation of enzymatic activity, enzyme reactions in heterogeneous systems.

Unit III Growth cycle, phases for Batch cultivation, mathematical modeling of batch growth, products synthesis Kinetics, overall kinetics and thermal death kinetics of cells and spores.

Unit IV Unit Operations in Biochemical Process: Agitation and aeration, gas liquid mass transfer, determination of oxygen transfer rates, determination of Kga and KLa scaling of mass transfer equipment, heat balance and heat transfer correlation for biochemical systems, sterilization, filtration and drying.

Unit V Design and Analysis of Bio-Reactors: Classification and characterization of different bioreactors, batch and continuous reactors, tubular, CSTR and tower reactors, aerobic and anaerobic fermentation-process, design and operation of typical aerobic and anaerobic fermentation processes, manufacture of microbial products e.g. antibiotics alcohol/ wine etc; use of immobilized enzyme and whole cells for industrial processes.

References:
1. Baily, J .E. and Ollis D.F; Biochemical Engineering Fundamentals; Mc. Graw Hill
2. Coulson and Richardson; Chemical Engineers;
3. Shuler, Kargi; Bioprocess Engineering basic concepts.; PHI Learning
4. Rao ; Introduction to Biochemical Engineering; TMH

List of Experiments(Please Expand It) Bio-Process Technology CM 803:
1. To carry out the isolation and identification of microorganism from a soil sample.
2. To examine & study effectiveness of various techniques for preserving microorganism
3. To study the kinetics of ethanol fermentation.
4. To determine the kinetic constants l.1max and Km for the growth of microorganisms.
5. To identify bacterial species using Gram staining tests.
6. To determine the biochemical oxygen demand of the given wastewater sample.
7. To determine the chemical oxygen demand of the given wastewater sample.
8. To study BOD kinetics of given wastewater sample and to determine the kinetic constant.
9. To determine the dissolved oxygen content of the given sample by Winkler method.
10. To determine the reducing sugar in the given fermentation medium.
11. To determine the protein in the given fermentation medium.
12. To determine the total sugar content in the given fermentation medium.
13. To study the kinetics of methane fermentation.
14. To study the kinetics of an enzyme catalyzed reaction.
15. To study the activity of enzymes in free and immobilized States.
16. To study the activity of whole cell enzymes in free and immobilized States.
Note: Each student should perform at least eight experiments out of the above list.

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